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1-10of13 Summaries or questions and answers
inconclusive
abbreviations
This product is known not to work in this application or species.
Application
Immunocytochemistry/Immunofluorescence
Sample
Human Cell (muscle)
permeabilization
Sim - PBS triton 0.5%
Specification
muscle
lock step
Serum as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 20% Temperature: 20°C
closing
paraformaldehyde
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The reviewer received a reward from the Abcam Loyalty Program as a thank you for submitting this abridged review and for helping the scientific community make more informed decisions.
Abcam user community
verified customer
Sent on March 14, 2016
Great
abbreviations
Application
western blot
amount of charge
20 µg
Gel operating conditions
Reduced denaturation (10%)
Sample
Human Tissue Lysate - Whole (Skeletal Muscle)
Specification
skeletal muscle
lock step
Milk as blocking agent for 2 hour(s) and 0 minute(s) Concentration: 5% Temperature: 25°C
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The reviewer received a reward from the Abcam Loyalty Program as a thank you for submitting this abridged review and for helping the scientific community make more informed decisions.
Dr. Rafael Montes Nieto
verified customer
Sent on October 31, 2014
Bom
abbreviations
Application
immunocytochemistry
lock step
FBS as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 22°C
Sample
Cultured human cells (immortalized myoblasts)
Specification
immortalized myoblasts
permeabilization
No
closing
paraformaldehyde
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The reviewer received a reward from the Abcam Loyalty Program as a thank you for submitting this abridged review and for helping the scientific community make more informed decisions.
Abcam user community
verified customer
Posted on September 5, 2014
Bom
abbreviations
Application
western blot
Sample
Mouse Tissue Lysate - Whole (Skeletal Muscle)
amount of charge
30 µg
Specification
skeletal muscle
Gel operating conditions
reduced denaturation
lock step
Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 25°C
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The reviewer received a reward from the Abcam Loyalty Program as a thank you for submitting this abridged review and for helping the scientific community make more informed decisions.
Abcam user community
verified customer
Posted on June 14, 2012
Bom
abbreviations
Application
Immunohistochemistry (frozen sections)
Sample
Rabbit tissue sections (esophagus)
Specification
Esophagus
closing
paraformaldehyde
lock step
Serum as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 10
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The reviewer received a reward from the Abcam Loyalty Program as a thank you for submitting this abridged review and for helping the scientific community make more informed decisions.
dr. Vyacheslav sabe
verified customer
Posted on May 15, 2007
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IHC-P mouse brain (2 different tumor types) without AR staining: pH 6 and 10 Ab: 1/100, 1/200 (5 ug/ml) - prediluted in the morning, used in the afternoon incubated for 1 h and the /n 2nd: AP , ABC - works fine, otherwise other primaries work fine
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abcam community
verified customer
Asked Feb 04 '13
Respondedor
I issued a free replacement with a new batch.
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Abcam Scientific Support
Answered on February 4, 2013
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I tested this anti-desmin antibody against IHC-Fr in mouse brain tissue, perfused with PFA, post-fixed in PFA for 1 hour, and cryoprotected in PB+sucrose. Stained with different protocols and concentrations (permeabilization with Trinton x 0.1-0.3% in 10% goat serum for 1h and concentrations ranging from 1:50 to 1:1500). I used different non-abcam gt anti-mouse secondary antibodies. I got staining that is not seen in controls without primary antibody, but is not as expected for desmin. It is the staining of the nucleus of some cells and it is supposed to be a cytoplasmic (intermediate filament) staining. He intended to stain blood vessels and pericytes with this antibody. I have an NG2 antibody that stains pericytes and I see them regularly on my stain. This was the first time I had used a desmin specific antibody. I have attached a photograph of the hippocampus (bottom), the corpus callosum (middle), and the deep layers of the cortex (top). It's labeling the pyramidal cell nuclei in the hippocampus, which was actually not expected. I'm sure because there are pericytes in the brain.
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abcam community
verified customer
Asked Nov 20 '12
Respondedor
Thank you for sending your protocol, which appears to be correct, and the image file. I agree that the antibody is staining something that is probably not desmin. Are you interested in testing a different antibody against desmin? I see that ab15200 was posted in an article that used it to identify pericytes:
Schadler KL et al. The delta-4 ligand plays a pivotal role in the formation of pericytes/vascular smooth muscle cells during vasculogenesis and expansion of tumor vessels in Ewing's sarcoma. Clin Cancer Res 16:848-56 (2010). PubMed: 20103680
Here is the link to the PubMed abstract:
http://www.ncbi.nlm.nih.gov/pubmed/20103680?dopt=Abstract
and a link to the antibody datasheet:
https://www.abcam.com/Desmin-anticuerpo-ab15200.html
I'll be happy to send it out as a free replacement for the ab6322 or a refund if you prefer. Please let me know how you would like to proceed.
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Abcam Scientific Support
Answered Nov 20 '12
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Dear Madam or Sir, I would like to request a quote for the following antibodies please: anti-desmin ab6322 antibody anti-desmin ab15200 antibody And I would like to ask your advice on how to choose between these two antibodies. Specifically, I'm going to test human hematopoietic progenitor cells. Do you have any information on which one will give a more specific color in this case? (The immunogen for polyclonal ab15200 is a human synthetic peptide, whereas for monoclonal ab6322 it is a full-length native pig protein). Any information will be greatly appreciated. Thank you very much for your attention and best regards,
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abcam community
verified customer
Asked on 10/06/2011
Respondedor
Thank you for your query, which has been forwarded to the scientific support team. I would like to assure you that all of our antibodies have been successfully tested and would be covered by our guarantee in the applications and species listed in the data sheets. Therefore, any Desmin antibodies tested on the species and application you are using should be suitable and warranted for your experiments. I am sorry to confirm that these antibodies have not been specifically tested in human hematopoietic progenitor cells. However, I am pleased to report that both ab15200 and ab6322 have been tested on human specimens and would be covered by our guarantee for use on specimens of this species. I can recommend checking the datasheet for a list of the tested apps to make sure the app you are using is also tested and warranted. Product prices can be found at the top of the data sheet, be sure to select Singapore from the country selection in the top right hand corner of the page to see the correct price. ab15200 Tested on: Flow Cyt, ICC/IF, IHC - Fr, IHC-P, IP, WB Price USD $365.00Click here(or use the following: https://www.abcam.com/Desmin-antibody-Cytoskeleton-Marker-ab15200.html).ab6322 tested on: ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB Price US$365.00Click here(or use the following: https://www.abcam.com/Desmin-antibody-DE-U-10-Cytoskeleton-Marker-ab6322.html), we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not be performed frequently. If you want to be more certain, I suggest you choose the antibody that has been best characterized. By that I mean the antibody that has been tested in the most applications and has the most data available (in the data sheet). I hope this helps. If you have any further questions, feel free to ask.
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Abcam Scientific Support
Answered Oct 6 '11
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I would like to test these antibodies in rhesus monkeys.
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abcam community
verified customer
Asked Sep 21 '11
Respondedor
Thank you for your call today. See below for trial discount codes and instructions for use. Get in touch if you have any questions! DISCOUNT CODES: (1) ab15463: *** (2) ab6322: *** Expiration Date: January 22, 2012 I am pleased that you would like to accept our offer and test these antibodies in rhesus monkeys. Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit a summary for each antibody in the rhesus samples and include the code in the "Additional Comments" section so we know the summary is for this promotion. Codes will be activated once the Abreviews are shipped and can be redeemed in one of the following ways: 1) call to place your order and mention the code to our customer service department; 2) Include the code in your fax request; 3) Place your order on the web and enter the promotional code. Any feedback you can provide will be greatly appreciated, be it positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abbreviews and wish you the best of luck in your search. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.
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Abcam Scientific Support
Answered Sep 21 '11
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I would like to know if it is possible to conjugate this antibody with FitC and what the cost would be.
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abcam community
verified customer
Asked Nov 30 '10
Respondedor
Unfortunately, we do not market this antibody or any other Desmin antibody in a conjugated version. I have no information at this time if and when a conjugated version will be added to the catalog.
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Abcam Scientific Support
Answered Nov 30 '10
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